When using a VIC labelled TaqMAN probe in a singleplex qPCR assay of a DNA target on an AB7900, the S-curves come up in both VIC (expected!) and FAM. I realise that this is to be expected due to the spectral overlap.
However, when a similar assay is carried out but the original source material was RNA based (and converted to cDNA), S-curves are only generated in the VIC channel with no "bleed" into FAM at all.
Can anyone offer an explanation of why this would be?
squallweatheredMember Since 02 Jul 2009
Offline Last Active Apr 23 2013 07:36 AM
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