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microfir

Member Since 26 Jun 2009
Offline Last Active Apr 03 2013 04:35 AM
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Posts I've Made

In Topic: Plasmid compatibly groups

06 February 2013 - 01:03 AM

I would like to use pMB1 based plasmid for protein expression under arabinose inducible promoter . And pBBR1 plasmid that carries lacZ under control of a specific promoter that should to be activated by the inducible protein. Can both of these plasmids reside in E. coli at the same time ?


Thanks

In Topic: Plasmid compatibly groups

05 February 2013 - 10:16 PM

With each other


In Topic: RNA integrity

21 August 2012 - 09:47 AM

Hi mike
I isolated RNA from various gram negative bacteria from all sort of physiological conditions (planktonic, biofilm, late stationery phase etc'), the only thing I can say for sure is that each bacteria has different "RNA pattern "(on gel and in biolanalyzer), some have more then one band (usually larger then 23S) some have different 16S:23S ratios.
Anyway your RNA doesn't look like it was degraded. It seems that part of your plot is lower then the base line (in the left part of the plot, right after the 23S peak). Maybe that’s the reason your cant get RIN value( ?)

Ofir

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