Thanks Kienow. Your method it looks very interesting, but unfortunately, I don't have enough time to do it. Anyway after some statistic's calculation I skipped this empty plasmid (positive control) and just normalized my data to the negative mimics.
ah good ! then I can skipped the data of this empty target gene then normalized my data to the negative mimics.
I have now five experiments what is the best calculation way to sum up my data ! is by taking the Median or the average !
Actually I did site directed mutagensis for my target genes by mutate the seed region, Luciferase assay shows miR-X upregualted the mutated target gene.
I used plightswitch_3UTR from SwitchGear Genomics. I used negative mimics as negative control !
my quiestion is if I can skip the data of empty vector and normalized my data according to the negative mimics !
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