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Hi evryone,
I'm doing a 5ml liquid culture of DH5 E.coli transformed with an ampicillin resistance plasmid. I'd like to have a culture that grew 12 to 16h in LB before doing the miniprep but that is really not fitting well my planning. I was wondering if it's OK to pause the culture by placing the tube at 4°C and then replace them at 37°C ?
WIll this step have any consequence on the bacterial growth or the plasmid expression?
Thanks in advance for your answers!
Sabrina
