3 replies to this topic

[hadrian123]

Hi,

Can someone tell me what is the blue, green and red-dye usually use in making ELISA sample buffer?

thanks

[sgt4boston]

These are just commercial dyes or food coloring. If you are making your own kit be sure to check for interference in your assay. The color is incorporated to help the user visualize buffers and conjugates added to the plate.

[Jo3y]

sgt4boston, on 03 September 2009 - 09:26 AM, said:

These are just commercial dyes or food coloring. If you are making your own kit be sure to check for interference in your assay. The color is incorporated to help the user visualize buffers and conjugates added to the plate.

Normally what kind of dyes used in the buffers and conjugates? How to determine whether there is any interference in the assay? Thank you.

[PAO_ahac]

Food coloring is used. To determine interference add the coloring at the lowest concentration just visible to the user to one component at a time and run that component (conjugate, wash, or buffer) with and without the additive in parallel with at least 2 dose response curves each and a few controls and or real samples Low Medium and High values ( you need to run a different matrix than the standards/calibrators. If the controls/samples do not produce the same concentrations then you have interference. The curves may be parallel with one another but if the controls do not shift to the same degree they will produce different values...thus interference.