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Zymography and Sucrose


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#1 Shan

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Posted 28 August 2009 - 02:24 PM

Hi everyone,

I need to run some zymography experiments soon and was looking up different protocols for it. The first one I read was from Current Protocols and in it's recipe, sucrose was included in setting up the gels. I was very curious and at the same time confused at why sucrose is important. I searched many other places and yet couldn't find an answer. Does anyone know about this? I really appreciate your help :P

Shan

P.S. by the way, other protocols that i read don't seem to have sucrose, so i guess it's dispensable?

Edited by Shan, 28 August 2009 - 02:25 PM.


#2 Shan

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Posted 01 September 2009 - 06:05 PM

Hmm....Seems nobody came across something similar in the recipe?




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