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Cell Culture and Pipette Tips


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4 replies to this topic

#1 borriello.87

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Posted 27 August 2009 - 04:01 PM

Hi all,
can anyone of you tell me if I have to use filter pipette tips or simply sterile pipette tips (without filter) for cell culture?

Thank you for your kindness.

#2 bob1

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Posted 27 August 2009 - 04:16 PM

Either should be fine so long as they are sterile. It is best to keep a set of pipettes for cell culture only.

#3 borriello.87

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Posted 27 August 2009 - 04:38 PM

Thank you bob1!
I think I'll use sterile pipette tips without filter because they are less expensive (as far as I know)...

#4 little mouse

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Posted 27 August 2009 - 10:43 PM

keep a set of pipettes for the cell culture and normal tips will be fine for most of your applications.
I only use filter tips for precious but potentially mycoplasma-contaminated human cells, and also in the P2 room where we culture cells with intracellular bacteria and virus.

#5 Felina

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Posted 13 January 2010 - 11:56 AM

To be on the safe side, I never touch my ongoing culture with any tip without filter; not eaven when taking cells for counting - who knows who has messed around with the pipette bevore. But once I seperated cells for my experiments from the main culture flask, I use the filterless tips (unless for very long term experiments)




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