Jump to content

  • Log in with Facebook Log in with Twitter Log in with Windows Live Log In with Google      Sign In   
  • Create Account

Submit your paper to J Biol Methods today!
Photo
- - - - -

how to combine several flow cytometry histogram figures?


  • Please log in to reply
3 replies to this topic

#1 Weijun

Weijun

    member

  • Members
  • Pip
  • 1 posts
0
Neutral

Posted 27 August 2009 - 07:22 AM

I did cell cycle synchronization and got wonderful histogram figures of different cell cycle phases. In most published papers the figure is combined histogram figures in Three-dimensional as attached picture. Does anyone know to make it? I guess I need some special software. who can tell me what is the name of this software?

Attached Thumbnails

  • Untitled_1_copy.jpg


#2 Carlton H

Carlton H

    Enthusiast

  • Active Members
  • PipPipPipPipPip
  • 95 posts
0
Neutral

Posted 31 August 2009 - 04:38 PM

You can't do that in excel? I was pretty sure you could. ... In any case, I know for a fact you can do it in GraphPad Prism. My girlfriend uses it and she LOVES it. It's pretty easy to use, too.

Cheers,
-Carlton
NextAdvance
Reliable laboratory instruments for the life sciences, designed to enable you to work more effectively and increase productivity. Check us out!

If my answers help you, please take a moment to check out our site and see if any of our products would help you as well! Thanks!

#3 Konstantinos Papadimitriou

Konstantinos Papadimitriou

    member

  • Members
  • Pip
  • 2 posts
0
Neutral

Posted 01 September 2009 - 04:33 AM

You can do it in excel using the transparent tool :rolleyes:

#4 pongser

pongser

    member

  • Members
  • Pip
  • 1 posts
0
Neutral

Posted 16 September 2009 - 09:44 AM

Hi,

Some of the standard acquisition programs of a flow cytometer allow such displays. For example all versions of CellQuest and CellQuest Pro from BD allow it (FACScan to FACS Calibur or Vantage). I am not sure about current Beckman Coulter programs, but their old acquisition programs (like Expo) allowed it too, so I guess their new protocols didn't degenerate in this aspect. Although BD did so. Their latest universal program FACS DiVa forgot this nice feature of its ancestors.

If you have some of the a.m. software and you need some more info about how to do it, let me know.

Regards

Jochen




Home - About - Terms of Service - Privacy - Contact Us

©1999-2013 Protocol Online, All rights reserved.