I want to concentrate protein in cell culture medium for western blot. Is there a simple method to do that?
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How to concentrate protein from culture medium
1 reply to this topic
Posted 19 May 2001 - 09:00 PM
Mix equal volume of your media with 20% ice cold trichloroacetic acid. Let stand in ice for 15 minutes. Centrifuge at 10000g for 10 minutes. Dry pellet in air and resuspend in 1/100th the volume of 15 mM Tris.HCl pH 7.8 ( 1/100the of the original media volume ).