5 replies to this topic

[vaccine]

Hi,

Has anyone ever used mouse plasma raised to their protein of interest as their primary antibody for staining cells for quick expression screening thru' immunofluorescence? If yes, do you dilute the plasma or use neat?

Thanks,
vaccine

[Carlton H]

vaccine, on Aug 27 2009, 09:12 AM, said:

Hi,

Has anyone ever used mouse plasma raised to their protein of interest as their primary antibody for staining cells for quick expression screening thru' immunofluorescence? If yes, do you dilute the plasma or use neat?

Thanks,
vaccine

I personally have never used plasma for staining but I've read papers where they have done it. The ranges of dilutions that I've seen range from 1:4 to over 1:100. I think 1:500 is the highest I've seen. I don't believe I ever read a protocol that called for using it neat.

Hope that helps.

-Carlton

[vaccine]

Carlton H, on Aug 31 2009, 05:04 PM, said:

vaccine, on Aug 27 2009, 09:12 AM, said:

Hi,

Has anyone ever used mouse plasma raised to their protein of interest as their primary antibody for staining cells for quick expression screening thru' immunofluorescence? If yes, do you dilute the plasma or use neat?

Thanks,
vaccine

I personally have never used plasma for staining but I've read papers where they have done it. The ranges of dilutions that I've seen range from 1:4 to over 1:100. I think 1:500 is the highest I've seen. I don't believe I ever read a protocol that called for using it neat.

Hope that helps.

-Carlton

[vaccine]

vaccine, on Aug 31 2009, 04:59 PM, said:

Carlton H, on Aug 31 2009, 05:04 PM, said:

vaccine, on Aug 27 2009, 09:12 AM, said:

Hi,

Has anyone ever used mouse plasma raised to their protein of interest as their primary antibody for staining cells for quick expression screening thru' immunofluorescence? If yes, do you dilute the plasma or use neat?

Thanks,
vaccine

I personally have never used plasma for staining but I've read papers where they have done it. The ranges of dilutions that I've seen range from 1:4 to over 1:100. I think 1:500 is the highest I've seen. I don't believe I ever read a protocol that called for using it neat.

Hope that helps.

-Carlton

Thank you

[bob1]

I've used serum but not plasma. Always diluted.

[vaccine]

bob1, on Sep 13 2009, 05:42 PM, said:

I've used serum but not plasma. Always diluted.

I see. I will give it a go.

Thanks