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plasma in immunoflourescence


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5 replies to this topic

#1 vaccine

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Posted 27 August 2009 - 05:12 AM

Hi,

Has anyone ever used mouse plasma raised to their protein of interest as their primary antibody for staining cells for quick expression screening thru' immunofluorescence? If yes, do you dilute the plasma or use neat?

Thanks,
vaccine

#2 Carlton H

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Posted 31 August 2009 - 04:04 PM

Hi,

Has anyone ever used mouse plasma raised to their protein of interest as their primary antibody for staining cells for quick expression screening thru' immunofluorescence? If yes, do you dilute the plasma or use neat?

Thanks,
vaccine


I personally have never used plasma for staining but I've read papers where they have done it. The ranges of dilutions that I've seen range from 1:4 to over 1:100. I think 1:500 is the highest I've seen. I don't believe I ever read a protocol that called for using it neat.

Hope that helps.

-Carlton
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#3 vaccine

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Posted 31 August 2009 - 04:59 PM

Hi,

Has anyone ever used mouse plasma raised to their protein of interest as their primary antibody for staining cells for quick expression screening thru' immunofluorescence? If yes, do you dilute the plasma or use neat?

Thanks,
vaccine


I personally have never used plasma for staining but I've read papers where they have done it. The ranges of dilutions that I've seen range from 1:4 to over 1:100. I think 1:500 is the highest I've seen. I don't believe I ever read a protocol that called for using it neat.

Hope that helps.

-Carlton



#4 vaccine

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Posted 12 September 2009 - 03:14 PM

Hi,

Has anyone ever used mouse plasma raised to their protein of interest as their primary antibody for staining cells for quick expression screening thru' immunofluorescence? If yes, do you dilute the plasma or use neat?

Thanks,
vaccine


I personally have never used plasma for staining but I've read papers where they have done it. The ranges of dilutions that I've seen range from 1:4 to over 1:100. I think 1:500 is the highest I've seen. I don't believe I ever read a protocol that called for using it neat.

Hope that helps.

-Carlton




Thank you

#5 bob1

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Posted 13 September 2009 - 04:42 PM

I've used serum but not plasma. Always diluted.

#6 vaccine

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Posted 14 September 2009 - 02:00 AM

I've used serum but not plasma. Always diluted.



I see. I will give it a go.

Thanks




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