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plasma in immunoflourescence

Started by vaccine, Aug 27 2009 05:12 AM

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5 replies to this topic

#1 vaccine

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Posted 27 August 2009 - 05:12 AM

Hi,

Has anyone ever used mouse plasma raised to their protein of interest as their primary antibody for staining cells for quick expression screening thru' immunofluorescence? If yes, do you dilute the plasma or use neat?

Thanks,
vaccine

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#2 Carlton H

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Posted 31 August 2009 - 04:04 PM

vaccine, on Aug 27 2009, 09:12 AM, said:

Hi,

Has anyone ever used mouse plasma raised to their protein of interest as their primary antibody for staining cells for quick expression screening thru' immunofluorescence? If yes, do you dilute the plasma or use neat?

Thanks,
vaccine

I personally have never used plasma for staining but I've read papers where they have done it. The ranges of dilutions that I've seen range from 1:4 to over 1:100. I think 1:500 is the highest I've seen. I don't believe I ever read a protocol that called for using it neat.

Hope that helps.

-Carlton

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#3 vaccine

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Posted 31 August 2009 - 04:59 PM

Carlton H, on Aug 31 2009, 05:04 PM, said:

vaccine, on Aug 27 2009, 09:12 AM, said:

Hi,

Has anyone ever used mouse plasma raised to their protein of interest as their primary antibody for staining cells for quick expression screening thru' immunofluorescence? If yes, do you dilute the plasma or use neat?

Thanks,
vaccine

I personally have never used plasma for staining but I've read papers where they have done it. The ranges of dilutions that I've seen range from 1:4 to over 1:100. I think 1:500 is the highest I've seen. I don't believe I ever read a protocol that called for using it neat.

Hope that helps.

-Carlton

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#4 vaccine

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Posted 12 September 2009 - 03:14 PM

vaccine, on Aug 31 2009, 04:59 PM, said:

Carlton H, on Aug 31 2009, 05:04 PM, said:

vaccine, on Aug 27 2009, 09:12 AM, said:

Hi,

Has anyone ever used mouse plasma raised to their protein of interest as their primary antibody for staining cells for quick expression screening thru' immunofluorescence? If yes, do you dilute the plasma or use neat?

Thanks,
vaccine

I personally have never used plasma for staining but I've read papers where they have done it. The ranges of dilutions that I've seen range from 1:4 to over 1:100. I think 1:500 is the highest I've seen. I don't believe I ever read a protocol that called for using it neat.

Hope that helps.

-Carlton

Thank you