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[ucsd]

Recently I overexpression a gene and knocking down the same gene in 3T3-L1 cells.
I check the mRNA level of this gene
I found the mRNA level of this gene in overexpression cell line did not go higher, just 1.1 folder higher than control. Is It possible?
I use retrovirus for infecting the 3T3-L1 cells and select the overexpression clones.

Also as for the knock down this gene by siRNA, I check two primer pairs,
one primer pair show the mRNA level in knocking down cells was 70% of control.
however another primer pair shows that mRNA level in knocking down cells was 30% of control.
Why the same cDNA but different primer giving me different knock down efficiency. 70% vs 30%, which one I should believe?

Thanks

Edited by ucsd, 25 August 2009 - 12:49 PM.