Jump to content

  • Log in with Facebook Log in with Twitter Log in with Windows Live Log In with Google      Sign In   
  • Create Account

Submit your paper to J Biol Methods today!
Photo
- - - - -

dehydration and rehydration of tissues


  • Please log in to reply
1 reply to this topic

#1 baboon

baboon

    member

  • Active Members
  • Pip
  • 10 posts
0
Neutral

Posted 25 August 2009 - 09:48 AM

Hi,

Can anyone explain me the principle of dehydrating and then rehydrating the tissues with methanol for IHC procedures.....

Thx.

#2 bob1

bob1

    Thelymitra pulchella

  • Global Moderators
  • PipPipPipPipPipPipPipPipPipPip
  • 5,525 posts
372
Excellent

Posted 25 August 2009 - 04:59 PM

The methanol binds to the water through van der Waals interactions and hydrogen bonding, lowering the evaporation point of the water resulting in dehydration of the tissue. There is also some osmotic effect going on - the water percent in methanol is usually quite low so the water in the cells gets sucked out to dilute the methanol, leading to further dehydration.

Rehydration has to take places to restore cells to their former size, the dehdration shrinks them.




Home - About - Terms of Service - Privacy - Contact Us

©1999-2013 Protocol Online, All rights reserved.