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I am just going to start CHIP expt for Histone acetylation study and this is the first time i will be doing this. There are 4 genes i am interested in. But i have no clue what i can expect about their acetylation level in patient samples.
I was thinking if i have any gene which always shows certain degree of H3K14/9 acetylation, then i can use that as positive control as in that case i already know i will get increased H3K14 acetylation in this gene. I tried to search for article, but could not yet.
I will really appreciate if you can tell me about a positive control i can use in CHIP H3K14 assay.
Thanks,
Edited by epigenetics, 25 August 2009 - 08:14 AM.
