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DyLight vs. IRDye Western Blot Li-Cor Odyssey


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#1 maho

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Posted 25 August 2009 - 06:04 AM

Which flourescent antibody works better on western blots with the Li-Cor Odyssey scanner? DyLight or IRDye labeled?

#2 haguilar

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Posted 10 September 2009 - 08:07 AM

Which flourescent antibody works better on western blots with the Li-Cor Odyssey scanner? DyLight or IRDye labeled?


I've never used the DyLight dyes with the odyssey, but Alexa-Fluor 680 (Molecular Probes) and IRDye800CW (LiCOR) work great (linear over a wide range). I wasn't even aware that you could use the DyLight's with the odyssey, though I suppose all you need is a dye that can be excited and specifically detected in the one channel by the scanner. You'd have to read into the spectral properties of the DyLight to be sure.




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