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RNA precipitation


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3 replies to this topic

#1 marianne

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Posted 09 March 2002 - 05:09 AM

i would like to precipitate again my RNA extracted from veray little tissu.
How can i make

#2 franck

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Posted 13 March 2002 - 01:15 AM

what is your tissue?

#3 sean

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Posted 15 March 2002 - 04:42 PM

rna ppt is very similar to dna ppt.

salt and ethanol are used and nucleic acid is allowed to ppt and spin and resuspend in a smaller volume.

look on current protocls or the web for RNA ppt methods.  but like i said it's similar to dna ppt.


#4 AronD

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Posted 16 March 2002 - 08:28 AM

If you try Sean's suggestion, you may also want to use glycogen to act as a carrier in the alcohol to help get the most RNA out.  It works pretty well and won't affect your RT-PCR.




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