3 replies to this topic

[marianne]

i would like to precipitate again my RNA extracted from veray little tissu.
How can i make

[franck]

what is your tissue?

[sean]

rna ppt is very similar to dna ppt.

salt and ethanol are used and nucleic acid is allowed to ppt and spin and resuspend in a smaller volume.

look on current protocls or the web for RNA ppt methods.  but like i said it's similar to dna ppt.

[AronD]

If you try Sean's suggestion, you may also want to use glycogen to act as a carrier in the alcohol to help get the most RNA out.  It works pretty well and won't affect your RT-PCR.