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transfection


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#1 anonymous

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Posted 22 August 2001 - 09:00 PM

I am interested in generating stable transfections in fibroblasts and squamous epithelial cells. Can anyone suggest what protocol and which reagents to use.

#2 anonymous

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Posted 23 August 2001 - 09:00 PM

Murali:

The protocol you need is in a paper in J. Cell Biology 1996. 134: 985-1001. Bornslaeger et al. and it starts out as Breaking the connection: displacement of the desmosomal plaque............etc. A lot of stable cell-line guys reference this article.

Good Luck

RahulDSTF-Global[url="http://www.dstf.bigstep.com"][url]http://www.dstf.bigstep.com[/url][/url]


#3 anonymous

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Posted 23 August 2001 - 09:00 PM

Murali:

The protocol you need is in a paper in J. Cell Biology 1996. 134: 985-1001. Bornslaeger et al. and it starts out as Breaking the connection: displacement of the desmosomal plaque............etc. A lot of stable cell-line guys reference this article.

Good Luck

RahulDSTF-Global[url="http://www.dstf.bigstep.com"][url]http://www.dstf.bigstep.com[/url][/url]





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