I am interested in generating stable transfections in fibroblasts and squamous epithelial cells. Can anyone suggest what protocol and which reagents to use.
Submit your paper to J Biol Methods today!
2 replies to this topic
#2
anonymous
-
-
- 1,890 posts
Veteran
0
Neutral
Neutral
Posted 23 August 2001 - 09:00 PM
Murali:
The protocol you need is in a paper in J. Cell Biology 1996. 134: 985-1001. Bornslaeger et al. and it starts out as Breaking the connection: displacement of the desmosomal plaque............etc. A lot of stable cell-line guys reference this article.
Good Luck
RahulDSTF-Global[url="http://www.dstf.bigstep.com"][url]http://www.dstf.bigstep.com[/url][/url]
#3
anonymous
-
-
- 1,890 posts
Veteran
0
Neutral
Neutral
Posted 23 August 2001 - 09:00 PM
Murali:
The protocol you need is in a paper in J. Cell Biology 1996. 134: 985-1001. Bornslaeger et al. and it starts out as Breaking the connection: displacement of the desmosomal plaque............etc. A lot of stable cell-line guys reference this article.
Good Luck
RahulDSTF-Global[url="http://www.dstf.bigstep.com"][url]http://www.dstf.bigstep.com[/url][/url]
