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Blurred low molecular weight bands in SDS-PAGE

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#1 HUD



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Posted 24 August 2009 - 09:11 AM

Hello Friends,

I cast and run 12% SDS-PAGE gels for plant protein extracts. Low molecular weight proteins bands of my gels are always blurred while the high MW bands appear fine. This effect is common for pre-stained MW markers too.
I'm wondering whether my gels are bad. I always allow gels to polymerize overnight at room temperature. Could this be a problem with insufficient amount of TEMED and/or ammonium persulfate?
I greatly appreciate your suggestions and comments.


Edited by HUD, 24 August 2009 - 09:12 AM.

#2 mdfenko


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Posted 24 August 2009 - 10:06 PM

no. it is just the nature of electrophoresis (it may also be caused by insufficient stacking, buffers being "off",...)

there are a couple of ways to combat this.

one is to use a gradient gel (5-15%, 10-20%, 10-15%,...).

another is to add urea to the formula.

i prefer gradients.
talent does what it can
genius does what it must
i do what i get paid to do

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