I cast and run 12% SDS-PAGE gels for plant protein extracts. Low molecular weight proteins bands of my gels are always blurred while the high MW bands appear fine. This effect is common for pre-stained MW markers too.
I'm wondering whether my gels are bad. I always allow gels to polymerize overnight at room temperature. Could this be a problem with insufficient amount of TEMED and/or ammonium persulfate?
I greatly appreciate your suggestions and comments.
Edited by HUD, 24 August 2009 - 09:12 AM.