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Is it possible to reuse electroporation cuvette to transfect siRNA


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4 replies to this topic

#1 labtek

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Posted 24 August 2009 - 06:55 AM

Is it possible to reuse electroporation cuvette to transfect siRNA? How to clean the cuvette for reuse? how to get rid of RNase if there is RNase contamination?

Thanks.

#2 miRNA man

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Posted 24 August 2009 - 10:25 AM

I'm not sure why you would want to re-use the cuvette. To save money? If so, why would you want to risk contamination and losing your experiment for a cuvette which probably costs no more than a couple of dollars?

#3 dvddecarvalho

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Posted 24 August 2009 - 03:08 PM

Rinse it with 70% ETOH (many times)
Let it under UV, about 1 hour
Pack in a plastic bag, seal it
Put it in microwave, put a becher with some water also,
Run about 10 minutes

That`s fine.
Use it with no fear.

You just can not reuse many times because the electrodes starts to deteriorate.
I reuse them like 3 times.

#4 labtek

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Posted 24 August 2009 - 06:24 PM

Thanks, dvddecarvalho.

#5 tfitzwater

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Posted 25 August 2009 - 06:50 AM

Drop the used cuvettes into a container of water to prevent the DNA and cells from drying out before you have time to clean them.




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