4 replies to this topic

[labtek]

Is it possible to reuse electroporation  cuvette to transfect siRNA? How to clean the cuvette for reuse? how to get rid of RNase if there is RNase contamination?

Thanks.

[miRNA man]

I'm not sure why you would want to re-use the cuvette. To save money? If so, why would you want to risk contamination and losing your experiment for a cuvette which probably costs no more than a couple of dollars?

[dvddecarvalho]

Rinse it with 70% ETOH (many times)
Let it under UV, about 1 hour
Pack in a plastic bag, seal it
Put it in microwave, put a becher with some water also,
Run about 10 minutes

That`s  fine.
Use it with no fear.

You just can not reuse many times because the electrodes starts to deteriorate.
I reuse them like 3 times.

[labtek]

Thanks, dvddecarvalho.

[tfitzwater]

Drop the used cuvettes into a container of water to prevent the DNA and cells from drying out before you have time to clean them.