Hello!
could someone tell me how to reuse my Ni NTA beads with the same protein? I want to get rid of the imidazole! I was told that I just had to wash 20% EtOH (for storage) away with water and then with buffer and I could use them again?
Thank you!
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2 replies to this topic
#2
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Enthusiast
Posted 24 August 2009 - 04:14 AM
PhD, on Aug 24 2009, 02:29 AM, said:
Hello!
could someone tell me how to reuse my Ni NTA beads with the same protein? I want to get rid of the imidazole! I was told that I just had to wash 20% EtOH (for storage) away with water and then with buffer and I could use them again?
Thank you!
could someone tell me how to reuse my Ni NTA beads with the same protein? I want to get rid of the imidazole! I was told that I just had to wash 20% EtOH (for storage) away with water and then with buffer and I could use them again?
Thank you!
20% EtOH wash and then PBS will be fine
#3
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an elder
Posted 24 August 2009 - 10:18 PM
check the handbook (look up "histrap" or "nickel"):
affinity chromatography handbook, ge healthcare
or check the novagen handbook:
novagen (merck) ni-nta handbook (pdf)
affinity chromatography handbook, ge healthcare
or check the novagen handbook:
novagen (merck) ni-nta handbook (pdf)
Edited by mdfenko, 24 August 2009 - 10:22 PM.
talent does what it can
genius does what it must
i do what i get paid to do
genius does what it must
i do what i get paid to do
