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reusing Ni NTA


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#1 PhD

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Posted 24 August 2009 - 02:29 AM

Hello!
could someone tell me how to reuse my Ni NTA beads with the same protein? I want to get rid of the imidazole! I was told that I just had to wash 20% EtOH (for storage) away with water and then with buffer and I could use them again?

Thank you!

#2 pesji

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Posted 24 August 2009 - 04:14 AM

Hello!
could someone tell me how to reuse my Ni NTA beads with the same protein? I want to get rid of the imidazole! I was told that I just had to wash 20% EtOH (for storage) away with water and then with buffer and I could use them again?

Thank you!

20% EtOH wash and then PBS will be fine :lol:

#3 mdfenko

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Posted 24 August 2009 - 10:18 PM

check the handbook (look up "histrap" or "nickel"):
affinity chromatography handbook, ge healthcare

or check the novagen handbook:
novagen (merck) ni-nta handbook (pdf)

Edited by mdfenko, 24 August 2009 - 10:22 PM.

talent does what it can
genius does what it must
i do what i get paid to do




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