2 replies to this topic

[PhD]

Hello!
could someone tell me how to reuse my Ni NTA beads with the same protein? I want to get rid of the imidazole!  I was told that I just had to wash 20% EtOH (for storage) away with water and then with buffer and I could use them again?

Thank you!

[pesji]

PhD, on Aug 24 2009, 02:29 AM, said:

Hello!
could someone tell me how to reuse my Ni NTA beads with the same protein? I want to get rid of the imidazole!  I was told that I just had to wash 20% EtOH (for storage) away with water and then with buffer and I could use them again?

Thank you!

20% EtOH wash and then PBS will be fine  

[mdfenko]

check the handbook (look up "histrap" or "nickel"):
affinity chromatography handbook, ge healthcare

or check the novagen handbook:
novagen (merck) ni-nta handbook (pdf)

Edited by mdfenko, 24 August 2009 - 10:22 PM.