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[autophagator]

Hello¡¡

In my last western of a small protein (16Kd), I filled the tank’s compartments with running buffer until the top of apparatus (the lower and upper buffer chambers, with the same level of liquid). When I revealed my gel, the bands appears diffuse and with a long black shadow.
I think that, because of there isn’t discontinuity in the buffer system; this might cause an electrical anomalous field, and therefore an anomalous migration of the proteins.
It is true or I am hallucinating?

Any suggestion will be welcome.
Thanks a lot.

[bob1]

It is unlikely to be that you caused an anomalous field, unless there was current leakage between the two chambers. If that had happened, your gel wouldn't have run, or would have run a lot slower than usual.