I plan to clone my predicted pre-miRNA. I will use miRNASelect™ pEGP-miR Cloning and Expression Vector.
Anyone have suggestion and commnet about these plasmid.
Thank.
piggle
0
Have anyone ever to use miRNASelect™ pEGP-miR Cloning and Expression Vector (CEL
Started by piggle, Aug 22 2009 11:55 PM
1 reply to this topic
#1
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Posted 22 August 2009 - 11:55 PM
Hi, all
I plan to clone my predicted pre-miRNA. I will use miRNASelect™ pEGP-miR Cloning and Expression Vector.
Anyone have suggestion and commnet about these plasmid.
Thank.
piggle
I plan to clone my predicted pre-miRNA. I will use miRNASelect™ pEGP-miR Cloning and Expression Vector.
Anyone have suggestion and commnet about these plasmid.
Thank.
piggle
#2
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- Active Members
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- 118 posts
Veteran
Posted 23 August 2009 - 02:48 PM
pEGP-miR and pEP-miR cloning and expression vectors were generally copying from Biosettia. In pEP-miR, everything including promoter, intron, restriction sites, and miRNA sequences are identical. Then they just changed the intron and replaced the RFP with GFP but kept the same cloning sites to make pEGP-miR vector and cloned mouse miRNAs. The Biosettia's miRNA expression vector is lenti based, you can package the lentivirus or use for transient transfection. It works very well on my hands. Please see http://biosettia.com.../products-mirna and http://www.protocol-online.org/forums/inde...?showtopic=9039 for more info
Edited by Functional Screens, 23 August 2009 - 02:50 PM.
