Posted 22 August 2009 - 02:26 PM
Posted 22 August 2009 - 04:52 PM
How do you clean-up the DNA after the IP (spin columns, phenol:chloroform and ethanol precipitation, Fast ChIP, etc.)? How do you prepare the total chromatin samples prior to running PCR (do you follow the same clean-up protocol as your IPs) or if you don't clean them up, do you at least dilute them in a PCR compatible buffer prior running PCR?
Posted 22 August 2009 - 05:46 PM
Posted 23 August 2009 - 05:56 PM
My suggestion is to use a MinElute PCR clean-up kit (or other low retention/low capacity clean-up kit) to clean-up your DNA and skip the phenol:chloroform and ethanol precipitation altogether.