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RNase: RNA ratio


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#1 jmp1

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Posted 21 August 2009 - 12:58 PM

I am trying to make dsRNA using sense and antisense transcripts generated off the same plasmid. I want to anneal the transcripts and degrade any remaining ssRNA. I plan to use RNase A or RNase T1. Does anyone know the ratio of RNase to RNA that I should use?

Thanks!

#2 torte

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Posted 07 September 2009 - 05:30 AM

try 2ul when starting with 1-2ug of DNA tmplate




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