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[jmp1]

I am trying to make dsRNA using sense and antisense transcripts generated off the same plasmid.  I want to anneal the transcripts and degrade any remaining ssRNA.  I plan to use RNase A or RNase T1.  Does anyone know the ratio of RNase to RNA that I should use?

Thanks!

[torte]

try 2ul when starting with 1-2ug of DNA tmplate