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Purification of virus particles for analysis


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#1 lene

lene

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Posted 21 August 2009 - 02:55 AM

Hello!

For particle size analysis of virions I have to purify the samples and get rid of the sucrose, which was added for the purification step with gradient ultracentrifugation.
I tried different systems like dialysis, filtration, tca-precipitation, ... . The problem is that the virus particles are very sticky and sticks to the membranes, the walls of the tubes, etc. so that in the end after purification no virus is left. I started adding tween 20 for keeping the protein soluble, but it does not help that much.

Has anybody an idea how to purify the virus sample without loosing all the material? Some different inexpensive methods? And in addition the virus particles should stay in their native state.

Thanks, lene




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