Posted 20 August 2009 - 10:30 AM
Posted 20 August 2009 - 08:27 PM
You don't have to do cryosection. If you culture the cells on glass cell culture slides, you can wash, fix and stain (for example with DAPI and Phalloidin) the cells directly. Or you could simply look at the culture dish with a microscope, no need to fix etc.
Posted 21 August 2009 - 08:34 AM
unfortunately, the compound can also bind to cell membrane so you cannot see intracellular under microscopy.
Posted 21 August 2009 - 09:25 AM