Help with Northern Blotting
Posted 19 August 2009 - 02:03 PM
I am having problems with Northern Blotting of potato periderm RNA. I am using DIG labeled oligonucleotide probes and Roche's DIG Easy Hyb Granules. I believe the problem is with the hybridization, which I am doing overnight @ 50-60 C in tubes. I am also going to try using modified Church-type buffers instead of the Easy Hyb granules. Eventually, I wish to use these probes for ISH. I would appreciated any advice on this.
Posted 23 August 2009 - 04:37 PM
Posted 24 August 2009 - 06:45 AM
I have started using modified a Church type buffer now, so that I can add or subtract BSA, formamide, dextran sulfate, PEG and alter salt concentrations, etc.
Posted 24 August 2009 - 05:51 PM
Posted 25 August 2009 - 06:40 AM