Improving transient CHO protein expression
Posted 19 August 2009 - 08:43 AM
I have played around with ratios and have an optimal ratio (1:1 with 1ug DNA and 1ug MAX transfection reagent). I have also tried putting the cells in hypothermic conditions which appears to have increased the expression by almost double, but this still isn't quite enough.
Any other suggestions of something I can do (without extra cloning) to imrpove the expression of the cells?
Posted 19 August 2009 - 09:27 AM
Have you done any EGFP or beta-Gal transfection to test what % of transfection do you have?
Posted 20 August 2009 - 02:10 AM
We transfected with GFP, gave about a 60% transfection rate.
Anyone who has any expreience with mammalian cells can help though, I'm not just looking for tricks specific to CHOs. I will try anything that has been shown to work on mammalian cells.