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0.5% crystal violet


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#1 SF_HK

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Posted 19 August 2009 - 08:03 AM

Hi,

I have to make 0.5% crystal violet in 25% methanol. So, if I understand correct is the following calculation corect for making 10ml total volme:


1) 0.05g crystal violet
2) 2.5ml 25% methanol
3) 7.5ml water.

Or do i have to dissolve the crystal violet in 10ml of 25%methanol?

#2 almost a doctor

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Posted 19 August 2009 - 08:18 AM

Hi,

I have to make 0.5% crystal violet in 25% methanol. So, if I understand correct is the following calculation corect for making 10ml total volme:


1) 0.05g crystal violet
2) 2.5ml 25% methanol
3) 7.5ml water.

Or do i have to dissolve the crystal violet in 10ml of 25%methanol?



You need to add 0.05g crystal violet + 2.5ml 100% methanol + 7.5ml water. This way you'll have 0.5% crytal violet in 25% methanol.
Your calculations give 0.625% methanol.

#3 genehunter

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Posted 19 August 2009 - 10:12 AM

Dissolve crystal violet in methanol first, then add DW.




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