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# 0.5% crystal violet

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### #1 SF_HK

SF_HK

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Posted 19 August 2009 - 08:03 AM

Hi,

I have to make 0.5% crystal violet in 25% methanol.  So, if I understand correct is the following calculation corect for making 10ml total volme:

1) 0.05g crystal violet
2) 2.5ml 25% methanol
3) 7.5ml water.

Or do i have to dissolve the crystal violet in 10ml of 25%methanol?

### #2 almost a doctor

almost a doctor

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Posted 19 August 2009 - 08:18 AM

SF_HK, on Aug 19 2009, 05:03 PM, said:

Hi,

I have to make 0.5% crystal violet in 25% methanol.  So, if I understand correct is the following calculation corect for making 10ml total volme:

1) 0.05g crystal violet
2) 2.5ml 25% methanol
3) 7.5ml water.

Or do i have to dissolve the crystal violet in 10ml of 25%methanol?

You need to add 0.05g crystal violet + 2.5ml 100% methanol + 7.5ml water.   This way you'll have 0.5% crytal violet in 25% methanol.

### #3 genehunter

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Posted 19 August 2009 - 10:12 AM

Dissolve crystal violet in methanol first, then add DW.