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PFA fixing: just washing or quenching ?


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#1 jpclamme

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Posted 18 August 2009 - 09:57 AM

Hi,

I would like to know if after fixing with PFA you only wash with PBS or if you quench the PFA with something.
I also heard of using Tris HCl in PBS to readjust the PH.

Does anyone do this ?

Thanks,

JP

#2 miBunny

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Posted 18 August 2009 - 04:28 PM

I wash out or if feeling lazy (or needing to go home) I just dilute it. I stain in facs tubes and fix with around 100-200 uL. I then add FACs wash to the top of the tube and put in the fridge. Its good for at least 2-3 weeks.

I would advise against not diluting or washing out the PFA




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