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How to solve this western bolt problem?


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#1 mimiwill

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Posted 18 August 2009 - 09:02 AM

Hey guys,

I struggled with my western blot. It always appeared dumbbell-shape bands, especially the protein molecular weight below 55 kDa.
Could you tell me how to solve this problem? Caz this shape of protein band is very difficult to qutitate.
Thanks a lot~~ :)

#2 medchemgirl

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Posted 18 August 2009 - 11:56 AM

You should post the pic of ur blot.

Hey guys,

I struggled with my western blot. It always appeared dumbbell-shape bands, especially the protein molecular weight below 55 kDa.
Could you tell me how to solve this problem? Caz this shape of protein band is very difficult to qutitate.
Thanks a lot~~ :lol:



#3 minylim

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Posted 21 August 2009 - 01:18 PM

I guess it's the problem with transfer. I have a colleague had same problem, shorten the transfer time and check the current during transfer. (+load slightly less amount of ptn) Hope this will help.




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