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What would happen? sequencing question

Started by Kami23, Aug 18 2009 07:38 AM

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#1 Kami23

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Posted 18 August 2009 - 07:38 AM

What would happen if a sequencing reaction was left O/N in the PCR block at RT? Would this affect the trace?

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#2 Timmay

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Posted 18 August 2009 - 07:52 AM

Kami23, on Aug 18 2009, 05:38 PM, said:

What would happen if a sequencing reaction was left O/N in the PCR block at RT? Would this affect the trace?

Considering that I leave my sequencing products ON at 15° C without observing any decrease in trace quality, I think it shouldn't really matter. Might of course be dependent on your used chemicals, I use ABI's BigDye Terminator 1.1 for reference.

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#3 Kami23

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Posted 18 August 2009 - 08:17 AM

Timmay, on Aug 18 2009, 04:52 PM, said:

Kami23, on Aug 18 2009, 05:38 PM, said:

What would happen if a sequencing reaction was left O/N in the PCR block at RT? Would this affect the trace?

Considering that I leave my sequencing products ON at 15° C without observing any decrease in trace quality, I think it shouldn't really matter. Might of course be dependent on your used chemicals, I use ABI's BigDye Terminator 1.1 for reference.

Ok its using big Dye 3.1 if that helps