2 replies to this topic

[Bunsen Honeydew]

Hi,

I'm sorting some lymphocytes and need to get the total RNA from this.  Does anyone know roughly how much RNA you can expect from a million cells?

Thanks in advance,

Bunsen

[gfischer]

Bunsen Honeydew, on Aug 18 2009, 08:58 AM, said:

Hi,

I'm sorting some lymphocytes and need to get the total RNA from this.  Does anyone know roughly how much RNA you can expect from a million cells?

Thanks in advance,

Bunsen

This will vary based on cell type, method, and from prep to prep.  That said, I've done RNA isolation from a B-lymphocyte cell line and generally got quite high yields, on the order of 1 mg/mL.

[Bunsen Honeydew]

I'm working with CD4 cells and sadly not pulling out anywhere near as much as that - more blood and sorting needed!