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How many cells...


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#1 Bunsen Honeydew

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Posted 18 August 2009 - 05:58 AM

Hi,

I'm sorting some lymphocytes and need to get the total RNA from this. Does anyone know roughly how much RNA you can expect from a million cells?

Thanks in advance,

Bunsen

#2 gfischer

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Posted 18 August 2009 - 06:31 AM

Hi,

I'm sorting some lymphocytes and need to get the total RNA from this. Does anyone know roughly how much RNA you can expect from a million cells?

Thanks in advance,

Bunsen


This will vary based on cell type, method, and from prep to prep. That said, I've done RNA isolation from a B-lymphocyte cell line and generally got quite high yields, on the order of 1 mg/mL.
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#3 Bunsen Honeydew

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Posted 18 August 2009 - 06:36 AM

I'm working with CD4 cells and sadly not pulling out anywhere near as much as that - more blood and sorting needed! :)




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