Dear all,
Recently I have some colleague told me about an ascite tumor model for Hep3B human liver cancer cells by injecting the cells intraperitoneally to nude mice. It sounds very strange for me as Hep3B should be adhesion cells. Therefore, I would like to have you opinions on whether should ascite tumor only be performed for suspension cancer cells while adhesion cancer cells will develop into solid tumor i.p.? Is it possible for those adhesion cells that have already differentiated so much that they became largely adhesion-independent to have a chance to develope such ascite tumor? I don't think that should be a "standard" Hep3B" cell though? Many thanks.
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