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FPLC and Protein Chromatography


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11 replies to this topic

#1 Luria Bertani

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Posted 17 August 2009 - 12:55 PM

Hello,

I use FPLC quite a bit. I was just wondering if there were any good alternatives/competitors to GE Healthcare (aka Pharmacia) resins and FPLC columns. I've had fair experience with their systems, but their products are rather pricey and the over pressuring on some columns (especially when loading samples) is rather annoying.

So I want to look for some alternatives. Can you help?

#2 bob1

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Posted 17 August 2009 - 04:14 PM

Try Waters. They are pretty well known for this sort of thing, though mostly focussed on HPLC and LC sort of stuff.

#3 swanny

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Posted 17 August 2009 - 06:01 PM

What types of separations are you thinking of? Google the chemistry type and you should see whoever else makes similar resins, columns etc. You could also go to www.biocompare.com for a general search engine.

One brand that I used to use was POROS perfusion beads. Very high flow rates very sharp bands for the ion exchange columns.
Heart disease kills more women than breast cancer, but heart attack symptoms differ from men's symptoms. Get to know your heart... it could save your life.

#4 mdfenko

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Posted 17 August 2009 - 08:48 PM

you can check out bio-rad:
medium pressure columns
talent does what it can
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#5 Luria Bertani

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Posted 19 August 2009 - 05:26 AM

Hello again,

Thank you for the suggestions.
Sorbitech does columns, but not common resins.
http://www.sorbtech....conolineColumns

I'm just doing ion exchange and gel filtration for various proteins. HPLC sounds similar to FPLC, but I'm not sure that you can interchange the columns...

#6 swanny

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Posted 19 August 2009 - 05:35 PM

Hello again,

Thank you for the suggestions.
Sorbitech does columns, but not common resins.
http://www.sorbtech....conolineColumns

I'm just doing ion exchange and gel filtration for various proteins. HPLC sounds similar to FPLC, but I'm not sure that you can interchange the columns...

The main difference is the pressure that the system can handle. You can use HPLC columns on FPLC systems and vice versa, as long as you are aware of the baseline pressures of each system. What system are you using?
Heart disease kills more women than breast cancer, but heart attack symptoms differ from men's symptoms. Get to know your heart... it could save your life.

#7 Luria Bertani

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Posted 21 August 2009 - 05:19 AM

Oh, thank you.

I'm using AKTA FPLC manufactured from GE Healthcare.

#8 genehunter

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Posted 21 August 2009 - 07:14 AM

For gelfiltration and most large scale ion exchange columns that usually only involves low pressure operation, we always buy empty columns and resions and pack ourselves.

Edited by genehunter, 21 August 2009 - 07:29 AM.


#9 mdfenko

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Posted 21 August 2009 - 04:55 PM

Oh, thank you.

I'm using AKTA FPLC manufactured from GE Healthcare.

the akta is a biocompatible hplc. it can be used for both hplc and fplc columns.
talent does what it can
genius does what it must
i do what i get paid to do

#10 biotef

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Posted 24 August 2010 - 01:59 AM

Oh, thank you.

I'm using AKTA FPLC manufactured from GE Healthcare.

the akta is a biocompatible hplc. it can be used for both hplc and fplc columns.



Hello,

Did you find an alternative since i also am having same issue with ge column and resin too expensive. if you have please let me know.

regards,

#11 HomeBrew

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Posted 24 August 2010 - 01:37 PM

Varian is another manufacturer to look at...

#12 biotef

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Posted 24 August 2010 - 07:22 PM

Varian is another manufacturer to look at...


varian waters have ion exchange resins but i suppose only for analytical purpose. we require ion excahnge resins to perform protein purification. we have AKTA FPLC and are using sepharose fast flow resins. so anything compatible with AKTA and similar to sepharsoe fast flow is what i am lookign at.

thanks for your sugestions




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