11 replies to this topic

[Luria Bertani]

Hello,

I use FPLC quite a bit. I was just wondering if there were any good alternatives/competitors to GE Healthcare (aka Pharmacia) resins and FPLC columns. I've had fair experience with their systems, but their products are rather pricey and the over pressuring on some columns (especially when loading samples) is rather annoying.

So I want to look for some alternatives. Can you help?

[bob1]

Try Waters.  They are pretty well known for this sort of thing, though mostly focussed on HPLC and LC sort of stuff.

[swanny]

What types of separations are you thinking of? Google the chemistry type and you should see whoever else makes similar resins, columns etc. You could also go to www.biocompare.com for a general search engine.

One brand that I used to use was POROS perfusion beads. Very high flow rates very sharp bands for the ion exchange columns.

[mdfenko]

you can check out bio-rad:
medium pressure columns

[Luria Bertani]

Hello again,

Thank you for the suggestions.
Sorbitech does columns, but not common resins.
http://www.sorbtech....conolineColumns

I'm just doing ion exchange and gel filtration for various proteins. HPLC sounds similar to FPLC, but I'm not sure that you can interchange the columns...

[swanny]

Luria Bertani, on Aug 19 2009, 11:26 PM, said:

Hello again,

Thank you for the suggestions.
Sorbitech does columns, but not common resins.
http://www.sorbtech....conolineColumns

I'm just doing ion exchange and gel filtration for various proteins. HPLC sounds similar to FPLC, but I'm not sure that you can interchange the columns...

The main difference is the pressure that the system can handle. You can use HPLC columns on FPLC systems and vice versa, as long as you are aware of the baseline pressures of each system. What system are  you using?

[Luria Bertani]

Oh, thank you.

I'm using AKTA FPLC manufactured from GE Healthcare.

[genehunter]

For  gelfiltration and most large scale ion exchange columns that usually only involves low pressure operation, we always buy  empty columns and resions and pack ourselves.

Edited by genehunter, 21 August 2009 - 07:29 AM.

[mdfenko]

Luria Bertani, on Aug 21 2009, 09:19 AM, said:

Oh, thank you.

I'm using AKTA FPLC manufactured from GE Healthcare.

the akta is a biocompatible hplc. it can be used for both hplc and fplc columns.

[biotef]

mdfenko, on 21 August 2009 - 04:55 PM, said:

Luria Bertani, on Aug 21 2009, 09:19 AM, said:

Oh, thank you.

I'm using AKTA FPLC manufactured from GE Healthcare.

the akta is a biocompatible hplc. it can be used for both hplc and fplc columns.

Hello,

Did you find an alternative since i also am having same issue with ge column and resin too expensive. if you have please let me know.

regards,

[HomeBrew]

Varian is another manufacturer to look at...

[biotef]

HomeBrew, on 24 August 2010 - 01:37 PM, said:

Varian is another manufacturer to look at...

varian waters have ion exchange resins but i suppose only for analytical purpose. we require ion excahnge resins to perform protein purification. we have AKTA FPLC and are using sepharose fast flow resins. so anything compatible with AKTA and similar to sepharsoe fast flow is what i am lookign at.

thanks for your sugestions