Jump to content

  • Log in with Facebook Log in with Twitter Log in with Windows Live Log In with Google      Sign In   
  • Create Account

Submit your paper to J Biol Methods today!
Photo
- - - - -

RNA isolation


  • Please log in to reply
1 reply to this topic

#1 daixiaol

daixiaol

    member

  • Active Members
  • Pip
  • 6 posts
0
Neutral

Posted 25 March 2002 - 02:32 AM

hi, friends,
i am doing rtpcr.  i isolate the RNA from the mesenteric artery and vein.  (it is a long time for the tissue cleaning.)   i get the RNA, add RNA inhibitor and store into the -80'C freezer.  i take it out to do one-step RT-PCR, and then store the RNA into -80 again.  i could get the result at first and cannot get it anymore soon.  the RNA is so easy to degrade? need i change to two-step to get and store the stable cDNA first?
Thanks a lot.

#2 anonymous

anonymous

    Veteran

  • PipPipPipPipPipPipPipPipPipPip
  • 1,890 posts
1
Neutral

Posted 25 March 2002 - 10:56 AM

Repeated thawing may degrade RNA. Why don't you aliquot your tissue sample so each time just take one piece for RT-PCR?




Home - About - Terms of Service - Privacy - Contact Us

©1999-2013 Protocol Online, All rights reserved.