Posted 17 August 2009 - 03:18 AM
I got a PCR program that has as final extension step (at least I interpret it as extension step) 30 minutes at 60°C. Normally I use 10 minutes at 72°C...
So what is the aim of this long time at 60 degrees?
3 min 94°C
30 s 94°C
60s 72°C (loop back to step 2 30 times)
30 min 60°C
Thanks for any ideas...
One must presume that long and short arguments contribute to the same end. - Epicurus
...except casandra's that belong to the funniest, most interesting and imaginative (or over-imaginative?) ones, I suppose.
Posted 17 August 2009 - 06:07 AM
But why do you need annealing time to be for 90sec?, 30 sec is enough, right?
Would love to hear what others have to say.
Posted 17 August 2009 - 05:16 PM