Jump to content

  • Log in with Facebook Log in with Twitter Log in with Windows Live Log In with Google      Sign In   
  • Create Account

Submit your paper to J Biol Methods today!
Photo
- - - - -

FLAG Antibody conjugated with FITC


  • Please log in to reply
No replies to this topic

#1 Teagan27

Teagan27

    member

  • Active Members
  • Pip
  • 15 posts
0
Neutral

Posted 16 August 2009 - 10:51 PM

Hi,

I have purchased a Anti-FLAG Mouse antibody conjugated with FITC from Sigma. I intend to use it for confocal microscopy and western blotting. They have provided a proceedure for my microscopy work, however when i contacted them about the western blotting (which the product information specifically recommends) they have informed me that it has never been used in a western blotting application (atleast in published work or in their testing). I do not have the correct equipment to detect the FITC directly. So i was thinking of using a secondary detection system (such as Lumi-Light Plus Western Blotting Kit from Roche) which detects the primary mouse anti-body.

I am worried that the conjugated FITC may inhibit the binding of the secondary anti-body. I have found examples where people have had success using a secondary detection system with primary anti-bodies conjugated with FITC and it did not appear to have any effect on the binding of the secondary anti-body.

I was wondering if anyone else had used a conjugated anti-body in the same mannor and had any success?


Thanks




Home - About - Terms of Service - Privacy - Contact Us

©1999-2013 Protocol Online, All rights reserved.