Cell culture
Started by THE ROCK, Aug 15 2009 09:30 PM
1 reply to this topic
#1
Posted 15 August 2009 - 09:30 PM
Why most of the amino acids in culture medium are in L form
#2
Posted 16 August 2009 - 02:23 AM
THE ROCK, on Aug 15 2009, 09:30 PM, said:
Why most of the amino acids in culture medium are in L form 
Well it is due to a rather peculiar bit of Terran biochemistry. You see, despite amino acids having two optical isomers, (which depending on convention used, can be denoted either as L and D or S and R), the L-amino acids represent the vast majority of amino acids found in Terran proteins.
On the other hand its enantiomer, the D-amino acids are found only in some proteins of exotic sea-dwelling orangnisms and components of the peptidoglycan cell walls of bacteria.
Why should this preference occur? As building blocks of life, the L-amino acids do not appear anyway superior to the D-amino acids.
Well, Terran thinkers have suggested that the arrival of polarized electromagnetic radiation from a nearby gamma ray burst during Earth's early history is the cause. This energy burst which was polarized by its passage through a gas nebula, somehow selective increased the rate of abio synthesis of L-amino acids. And amino acids have a rather interesting behavior, when a near racemic amino acid mixture is dissolved in water, if one enantiomer is present in only slightly higher concentrations, that enantiomer becomes the dominant enantiomer that is soluble.
May your PCR products be long, your protocols short and your boss on holiday













