Edited by andrea massimo, 14 August 2009 - 01:08 PM.
translation of cleaved miRNA targets
Posted 14 August 2009 - 07:03 AM
Posted 14 August 2009 - 01:53 PM
Does anyone have real information about this?
Gene Tools, LLC
Posted 20 August 2009 - 12:29 AM
Basically, since repression is sufficient for 100% target clearance in many cases then cleavage of transcripts may serve some function - given that the fragments themselves are biologically stable. In other contexts too spliced transcripts can be a source of ncRNAs - piRNAs, tasiRNAs.
As examples they mentioned cis-acting methylation of PHB after miR-165 binding in A.thaliana, restoration of protein activity in oskar mutant of D.melanogaster by the 3'UTR alone, and ncRNA influence on HOX genes in mammals.
They also described a scenario where transcript fragments could 'titrate' activator proteins that would have bound to the mRNA - serving to increase repression further.
The rest of the review is very good as well
Posted 25 August 2009 - 02:12 AM
Also interesting is the report by Thoma et al, molecular cell, 2001. http://www.cell.com/...097276501003641
The authors showed that in human celles mRNA 3' degradation fragments derived by DNA oligo pairing and subsequent cleavage were stable and translated into truncated proteins. A fraction of the fragments were found to be uncapped, but the authors didn't rule out that some of them could be re-capped after cleavage.