Help me! How to design nested BSP primers?
Posted 14 August 2009 - 07:02 AM
I am now doing nested BSP, but i don't know how to design nested BSP primer, can you take an example to explain nested BSP primer design? So many people are willing to know the method, it is kindhearted for you to do so!
Posted 14 August 2009 - 02:33 PM
for a nested set you are designing a pair of internal primers to the original ones for your region of interest, then your perform two rounds of PCR, the first round with your outer primer set, then take an aliquot of that into your second round PCR using your internal primer set.
I tend to do hemi nested PCRs so I have one internal primer and repeat the second round with that and the opposing first round primer.
hope this explains things
Posted 17 August 2009 - 11:44 PM
Posted 01 September 2009 - 07:51 PM
Posted 03 April 2010 - 10:18 PM