2 replies to this topic

[SF_HK]

this may be a silly question.  When we do transient transfection of a plasmid using lipofectamine, is it transfected into the cytoplasm but what allows it to enter the nuclear membrane?  Likewise for luciferase reporter gene assays.

[autumn_lullaby]

Hey SF,

I don't think your Q is silly at all. It's good to be inquisitive. Well, I have found a pdf article from Invitrogen which explains a little bit on how lipid-mediated transfection works. So, hope it helps

Autumn

Attached Files

•  Lipid_Transfection.pdf   102.81K   80 downloads

[genehunter]

It is generally agreed that thye disruption and re-organization of nuclear envelope during cell division is responsible for the majority nuclear entry of transfected plasmid, although DNA-associated nuclear proteins have some implications in facilitated nuclear translocation, esp for shorter DNA fragments.