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transient transfection


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#1 SF_HK

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Posted 13 August 2009 - 07:26 PM

this may be a silly question. When we do transient transfection of a plasmid using lipofectamine, is it transfected into the cytoplasm but what allows it to enter the nuclear membrane? Likewise for luciferase reporter gene assays.

#2 autumn_lullaby

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Posted 17 August 2009 - 12:41 AM

Hey SF,

I don't think your Q is silly at all. It's good to be inquisitive. Well, I have found a pdf article from Invitrogen which explains a little bit on how lipid-mediated transfection works. So, hope it helps :P

Autumn

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#3 genehunter

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Posted 17 August 2009 - 06:24 AM

It is generally agreed that thye disruption and re-organization of nuclear envelope during cell division is responsible for the majority nuclear entry of transfected plasmid, although DNA-associated nuclear proteins have some implications in facilitated nuclear translocation, esp for shorter DNA fragments.




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