CHO or VERO?
Posted 13 August 2009 - 10:21 AM
Posted 13 August 2009 - 04:18 PM
Posted 14 August 2009 - 05:48 AM
I have tired to express in bacteria and have had little luck. Granted I have not tried too hard. It is a small, highly folded protein. We have made modifications to avoid signal cleavage and secretion. I have performed Westerns on cells and sup (just in case it was secreted). Hoping all mammalian cells will work better for expression.