2 replies to this topic

[AReslow]

Trying to express fusion protein.  I have searched ATCC extensively.  Just need a simple answer- which is preferred, easiest to work with?

Thanks!

[bob1]

both are easy to work with, and should be fine for this sort of thing.  Having said that, if you want to purify the protein, express it in bacteria.

[AReslow]

Thanks Bob,

I have tired to express in bacteria and have had little luck.  Granted I have not tried too hard.  It is a small, highly folded protein.  We have made modifications to avoid signal cleavage and secretion.  I have performed Westerns on cells and sup (just in case it was secreted).  Hoping all mammalian cells will work better for expression.