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ELISA kit detection B. cereus toxins


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#1 pitoe

pitoe

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Posted 13 August 2009 - 04:37 AM

I am looking for a way to detect a low quantity of Bacillus cereus (contamination) in a vaccine batch. The toxin which I want to detect is the Bacillus diarrhoeal enteretoxin (BDE). I have found some ELISA kits online that can detect this toxin in food with a sensitivity of 1ng/ml (how does this compare to the average ELISA sensitivity?) sample but can this kit also be used for vaccine samples? I also came across the reverse passive latex agglutination (RPLA) method for detecting the toxin. Does anyone know how this works and its suitabilty for vaccine samples? Any help would be appreciated, thanks!




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