Hi,
Does anybody know the protocol of Hammerhead ribozyme clevage?and how to design the ribozyme?
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Hammerhead ribozyme cleavage
Started by bluebird, Aug 13 2009 04:13 AM
2 replies to this topic
#2
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Posted 13 August 2009 - 06:41 AM
Sorry I don't have much. Here's a paper on inhibiting a ribozyme; the references might be useful for design.
Yen L, Svendsen J, Lee JS, Gray JT, Magnier M, Baba T, D'Amato RJ, Mulligan RC. Exogenous control of mammalian gene expression through modulation of RNA self-cleavage. Nature. 2004 Sep 23;431(7007):471-6.
Good hunting,
- Jon
Yen L, Svendsen J, Lee JS, Gray JT, Magnier M, Baba T, D'Amato RJ, Mulligan RC. Exogenous control of mammalian gene expression through modulation of RNA self-cleavage. Nature. 2004 Sep 23;431(7007):471-6.
Good hunting,
- Jon
Jon D. Moulton
Gene Tools, LLC
www.gene-tools.com
Gene Tools, LLC
www.gene-tools.com
#3
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Posted 16 August 2009 - 12:16 PM
Let's make it clear. Ur trying to get a hyammerhead ribozyme. Is that right?
What I know is you can use SELEX to screen it. I don't know how to design it.
What I know is you can use SELEX to screen it. I don't know how to design it.
