Jump to content

  • Log in with Facebook Log in with Twitter Log in with Windows Live Log In with Google      Sign In   
  • Create Account

Submit your paper to J Biol Methods today!
Photo

Hammerhead ribozyme cleavage


  • Please log in to reply
2 replies to this topic

#1 bluebird

bluebird

    Enthusiast

  • Active Members
  • PipPipPipPipPip
  • 33 posts
0
Neutral

Posted 13 August 2009 - 04:13 AM

Hi,

Does anybody know the protocol of Hammerhead ribozyme clevage?and how to design the ribozyme?

#2 Jon Moulton

Jon Moulton

    Veteran

  • Active Members
  • PipPipPipPipPipPipPipPipPipPip
  • 137 posts
3
Neutral

Posted 13 August 2009 - 06:41 AM

Sorry I don't have much. Here's a paper on inhibiting a ribozyme; the references might be useful for design.

Yen L, Svendsen J, Lee JS, Gray JT, Magnier M, Baba T, D'Amato RJ, Mulligan RC. Exogenous control of mammalian gene expression through modulation of RNA self-cleavage. Nature. 2004 Sep 23;431(7007):471-6.

Good hunting,

- Jon
Jon D. Moulton
Gene Tools, LLC
www.gene-tools.com

#3 hei2zhu

hei2zhu

    member

  • Active Members
  • Pip
  • 5 posts
0
Neutral

Posted 16 August 2009 - 12:16 PM

Let's make it clear. Ur trying to get a hyammerhead ribozyme. Is that right?
What I know is you can use SELEX to screen it. I don't know how to design it.




Home - About - Terms of Service - Privacy - Contact Us

©1999-2013 Protocol Online, All rights reserved.