Hammerhead ribozyme cleavage
Posted 13 August 2009 - 04:13 AM
Does anybody know the protocol of Hammerhead ribozyme clevage?and how to design the ribozyme?
Posted 13 August 2009 - 06:41 AM
Yen L, Svendsen J, Lee JS, Gray JT, Magnier M, Baba T, D'Amato RJ, Mulligan RC. Exogenous control of mammalian gene expression through modulation of RNA self-cleavage. Nature. 2004 Sep 23;431(7007):471-6.
Gene Tools, LLC
Posted 16 August 2009 - 12:16 PM
What I know is you can use SELEX to screen it. I don't know how to design it.