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TrypeLE and Fixing


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#1 jpclamme

jpclamme

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Posted 12 August 2009 - 01:43 PM

Hi,

I have an experiment were I detach my cells with TrypLE and after 5 minutes I add PBS.
After that I want to fix the cells with PFA and measure protein fluorescence expression by FACS (no Abs staining).

As I don't have much cells and wish to minimize lost through washing steps, I was wondering if I could fix the cells directly in the TrypLE/PBS solution without spining down and washing the cells. Then only do 1 wash after fixing.

Thanks for your help,

JP




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