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plasmid sequence identification problem


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#1 Omneya

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Posted 11 August 2009 - 10:05 PM

Dear Members,

I have a problem and I need help please. I was able to isolate to new unknown plasmids from halophilic bacteria and I was doing sequence of these plasmid by random DNA library construction. Now the sequence fragments of each plasmid doesnt show any overlap. I am nor sure what is the reason.
The purification was intensive to separate each plasmid but I would like to know if there is other possibilities for such a problem please.
I need your expert opinion as soon as possible.



Best Regards.
Omeya

#2 Omneya

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Posted 13 August 2009 - 08:23 PM

By the way I compared with all results by DNA alignment software GENETIX,and could not find any overlap.

If the someone knows better software or solution, please inform me soon.
Thank you ;)

#3 jennifer 18

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Posted 20 October 2009 - 06:19 AM

please check BxSeqTools at http://bioinfoman.com




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